Associations between obesity and vitamin D availability depending on the rs2228570 polymorphism of the VDR gene and rs9939609 polymorphism of the FTO gene in the midland and the Extreme North of Russia

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Abstract

Background: It has been shown that vitamin  D availability depends on the body mass index (BMI). Genetic polymorphisms contribute to the development of vitamin  D deficiency.

Aim: To study the availability of vitamin D in the population of various regions of the Russian Federation, depending on the BMI values and the rs2228570 polymorphisms of the VDR gene and rs9939609 of the FTO gene.

Materials and methods: The rs2228570 polymorphisms of the VDR gene and rs9939609 of the FTO gene were identified in 311 subjects (136, from the midland of Russia, and 175 from the Far North). Serum 25-hydroxyvitamin  D [25(OH)D] levels were measured by an immunoenzyme assay in the autumn and winter seasons. Genotyping was performed with the allele-specific amplification and real-time detection of results using TaqMan probes complementary to the polymorphic DNA segments and the CFX96 Real Time System amplifier (Bio-Rad, USA). We studied associations of the rs9939609 polymorphism of the fat mass and obesity-associated (FTO) gene located at 16q12.2, as well as the rs2228570 polymorphism of the vitamin D receptor gene (VDR) located at 12q13.11.

Results: Frank vitamin D deficiency (serum 25 (OH) D level<20 ng/ml) was observed in 39.7% (54/136) of the sample from the midland, in 40% (14/35) of the migrants and in 30.7% (43/140) of the indigenous inhabitants of the Far North (Nenets). Obese residents of the midland Russia had significantly lower serum 25(OH)D levels, and the indigenous population of the Far North had significantly higher levels than those with BMI<30 (р<0.05). In the indigenous population of the Northern Region, there was a  significant association between vitamin  D deficiency and C allele of the rs2228570 polymorphism of the VDR gene (odds ratio [OR] 2.5, 95% confidence interval [CI] 1.46–4.27, p=0.0006) and the AA genotype of the rs9939609 polymorphism of the FTO gene (OR 8.83, 95% CI 0.94–82.5, p=0.02).

Conclusion: The association between obesity and vitamin  D availability in the individuals with the rs2228570 polymorphism of the VDR gene and the rs9939609 polymorphism of the FTO gene depends on their ethnicity. 

About the authors

A. V. Pogozheva

Federal Research Centre of Nutrition and Biotechnology;
I.M. Sechenov First Moscow State Medical University

Author for correspondence.
Email: allapogozheva@yandex.ru

Alla V. Pogozheva – MD, PhD, Professor, Leading Research Fellow, Laboratory of Epidemiology of Nutrition and Genodiagnostics of Alimentary-dependent Diseases, Federal Research Centre of Nutrition and Biotechnology

2/14 Ust'inskiy proezd, Moscow, 109240

Professor, Department of Food Hygiene and Toxicology, I.M. Sechenov First Moscow State Medical University

8/2 Trubetskaya ul., Moscow, 119991

Russian Federation

E. Yu. Sorokina

Federal Research Centre of Nutrition and Biotechnology;
I.M. Sechenov First Moscow State Medical University

Email: fake@neicon.ru

Elena Yu. Sorokina – MD, PhD, Leading Research Fellow, Laboratory of Epidemiology of Nutrition and Genodiagnostics of Alimentary-dependent Diseases, Federal Research Centre of Nutrition and Biotechnology

2/14 Ust'inskiy proezd, Moscow, 109240;

Associate Professor, Department of Food Hygiene and Toxicology, I.M. Sechenov First Moscow State Medical University

8/2 Trubetskaya ul., Moscow, 119991 

Russian Federation

A. A. Sokolnikov

Federal Research Centre of Nutrition and Biotechnology

Email: fake@neicon.ru

Andrey A. Sokolnikov – PhD (in Biology), Senior Research Fellow, Clinic,

2/14 Ust'inskiy proezd, Moscow, 109240

Russian Federation

References

  1. Kılıç S, Sılan F, Hız MM, Işık S, Ögretmen Z, Özdemir Ö. Vitamin D receptor gene BSMI, FOKI, APAI, and TAQI polymorphisms and the risk of atopic dermatitis. J Investig Allergol Clin Immunol. 2016;26(2):106–10. doi: 10.18176/jiaci.0020.
  2. Gilbert R, Bonilla C, Metcalfe C, Lewis S, Evans DM, Fraser WD, Kemp JP, Donovan JL, Hamdy FC, Neal DE, Lane JA, Smith GD, Lathrop M, Martin RM. Associations of vitamin D pathway genes with circulating 25-hydroxyvitamin-D, 1,25-dihydroxyvitamin-D, and prostate cancer: a nested case-control study. Cancer Causes Control. 2015;26(2):205–18. doi: 10.1007/s10552-014-0500-5.
  3. Hossein-Nezhad A, Eshaghi SM, Maghbooli Z, Mirzaei K, Shirzad M, Curletto B, Chen TC. The role of vitamin D deficiency and vitamin D receptor genotypes on the degree of collateralization in patients with suspected coronary artery disease. Biomed Res Int. 2014;2014:304250. doi: 10.1155/2014/304250.
  4. Jakubowska-Pietkiewicz E, Młynarski W, Klich I, Fendler W, Chlebna-Sokół D. Vitamin D receptor gene variability as a factor influencing bone mineral density in pediatric patients. Mol Biol Rep. 2012;39(5):6243–50. doi: 10.1007/s11033-012-1444-z.
  5. Wang G, Zhang Q, Xu N, Xu K, Wang J, He W, Yang T. Associations between two polymorphisms (FokI and BsmI) of vitamin D receptor gene and type 1 diabetes mellitus in Asian population: a meta-analysis. PLoS One. 2014;9(3):e89325. doi: 10.1371/journal.pone.0089325.
  6. Каронова ТЛ, Гринева ЕН, Михеева ЕП, Беляева ОД, Красильникова ЕИ, Никитина ИЛ. Уровень витамина D и его взаимосвязь с количеством жировой ткани и содержанием адипоцитокинов у женщин репродуктивного возраста. Проблемы эндокринологии. 2012;58(6):19–23.
  7. Vimaleswaran KS, Berry DJ, Lu C, Tikkanen E, Pilz S, Hiraki LT, Cooper JD, Dastani Z, Li R, Houston DK, Wood AR, Michaëlsson K, Vandenput L, Zgaga L, Yerges-Armstrong LM, McCarthy MI, Dupuis J, Kaakinen M, Kleber ME, Jameson K, Arden N, Raitakari O, Viikari J, Lohman KK, Ferrucci L, Melhus H, Ingelsson E, Byberg L, Lind L, Lorentzon M, Salomaa V, Campbell H, Dunlop M, Mitchell BD, Herzig KH, Pouta A, Hartikainen AL; Genetic Investigation of Anthropometric Traits-GIANT Consortium, Streeten EA, Theodoratou E, Jula A, Wareham NJ, Ohlsson C, Frayling TM, Kritchevsky SB, Spector TD, Richards JB, Lehtimäki T, Ouwehand WH, Kraft P, Cooper C, März W, Power C, Loos RJ, Wang TJ, Järvelin MR, Whittaker JC, Hingorani AD, Hyppönen E. Causal relationship between obesity and vitamin D status: bi-directional Mendelian randomization analysis of multiple cohorts. PLoS Med. 2013;10(2):e1001383. doi: 10.1371/journal.pmed.1001383.
  8. Turer CB, Lin H, Flores G. Prevalence of vitamin D deficiency among overweight and obese US children. Pediatrics. 2013;131(1):e152–61. doi: 10.1542/peds.2012-1711.
  9. Larcombe L, Mookherjee N, Slater J, Slivinski C, Dantouze J, Singer M, Whaley C, Denechezhe L, Matyas S, Decter K, Turner-Brannen E, Ramsey C, Nickerson P, Orr P. Vitamin D, serum 25(OH)D, LL-37 and polymorphisms in a Canadian First Nation population with endemic tuberculosis. Int J Circumpolar Health. 2015;74(1):28952. doi: 10.3402/ijch.v74.28952.
  10. Lourenço BH, Qi L, Willett WC, Cardoso MA; ACTION Study Team. FTO genotype, vitamin D status, and weight gain during childhood. Diabetes. 2014;63(2):808–14. doi: 10.2337/db13-1290.
  11. Батурин АК, Погожева АВ, Кешабянц ЭЭ, Старовойтов МЛ, Кобелькова ИВ, Камбаров АО. Изучение питания, антропометрических показателей и состава тела у коренного и пришлого населения российской Арктики. Вопросы питания. 2017;86(5):12–8. doi: 10.24411/0042-8833-2017-00070.
  12. Козлов АИ, Вершубская ГГ, Негашева МА. Полиморфизм гена рецептора витамина D (VDR) в выборках населения Европейской России и Приуралья. Пермский медицинский журнал. 2016;33(5):60–6. doi: 10.17816/pmj33560-66.
  13. Osman E, Al Anouti F, El Ghazali G, Haq A, Mirgani R, Al Safar H. Frequency of rs731236 (Taql), rs2228570 (Fok1) of Vitamin-D Receptor (VDR) gene in Emirati healthy population. Meta Gene. 2015;6:49–52. doi: 10.1016/j.mgene.2015.09.001.
  14. Батурин АК, Сорокина ЕЮ, Погожева АВ, Кешабянц ЭЭ, Кобелькова ИВ, Камбаров АО, Елизарова ЕВ, Тутельян ВА. Изучение ассоциации полиморфизмов rs993609 гена FTO и rs659366 гена UCP2 с ожирением у населения Арктической зоны Российской Федерации. Вопросы питания. 2017;86(3):32–9. doi: 10.24411/0042-8833-2017-00042.
  15. Harbron J, van der Merwe L, Zaahl MG, Kotze MJ, Senekal M. Fat mass and obesity-associated (FTO) gene polymorphisms are associated with physical activity, food intake, eating behaviors, psychological health, and modeled change in body mass index in overweight/ obese Caucasian adults. Nutrients. 2014;6(8): 3130–52. doi: 10.3390/nu6083130.

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Copyright (c) 2019 Pogozheva A.V., Sorokina E.Y., Sokolnikov A.A.

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