Evaluation of microRNA profile in cervical epithelium for predicting cervical cancer recurrence

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Abstract

Background: To predict the development and recurrence of cervical cancer (CC), we selected three oncoassociated miRNAs: miRNA-20a, -21, whose overexpression leads to the development of tumors, and -23b, which acts as an oncosuppressor. Aim: To evaluate the microRNA profile in the cervical epithelium for predicting CC recurrence in patients who underwent early treatment.

Materials and methods: In the study of the informativeness of expression included 145 patients with T1a1-T2a1N0M0 CC who were followed up for 2 years after treatment. Expression of microR-NA-20a, -21 and -23b was analyzed in tumor tissue samples.

Results: The risk of recurrence decreased from 1.0 to 0.92 after 1 year of the follow-up, and to 0.84 after 2 years. The initial expression of microRNA-20a and -21 in the cervical epithelium in patients with recurrent CC was 44% and 47% higher, respectively, than in patients without recurrence, while microRNA-23b expression was 46% lower. When initial levels of microRNA-20a and -21 expressions were 1.08 and 1.18, respectively, the risk of CC recurrence during the first two years after the surgery increased by 10.15 and 7.62 times, respectively. MicroRNA-20a expression in cervical epithelium equal to 1.08 was associated with 23% risk, and equal to 1.4 - with 79.7% risk. MicroRNA-21 expression equal to 1.18 was associated with 15% risk of CC recurrence; equal to 1.4 - with 55.5% risk; equal to 1.7 - 94.6%. Logistic regression showed that recurrence risks increased sharply when microRNA-23b expression declined.

Conclusion: We registered higher levels of mi-croRNA-20a and -21 expressions and lower mi-croRNA-23b expression in patients with recurrent CC, compared to favorable course of the disease. An analysis of the expression profiles of micro-RNA-20a, -21 and -23b after CC diagnosis allow prognosis of recurrence risks within 2 years after the tumor removal surgery.

About the authors

A. Yu. Maksimov

National Medical Research Centre for Oncology

Author for correspondence.
Email: onko-sekretar@mail.ru
ORCID iD: 0000-0002-1397-837X

Aleksey Yu. Maksimov - MD, PhD, Professor, Deputy Director.

63 14-ya liniya, Rostov-on-Don, 344037; Tel.: +7 (863) 200 10 00; +7 (863) 300 02 00

Russian Federation

M. Yu. Timoshkova

National Medical Research Centre for Oncology

Email: m-timoshkova@yandex.ru
ORCID iD: 0000-0003-1484-0580

Maria Yu. Timoshkova - MD, Oncologist, Junior Research Fellow, Experimental Laboratory Center.

63 14-ya liniya, Rostov-on-Don, 344037; Tel.: +7 (960) 489 80 80

Russian Federation

E. V. Verenikina

National Medical Research Centre for Oncology

Email: ekat.veren@yandex.ru
ORCID iD: 0000-0002-1084-5176

Ekaterina V. Verenikina - MD, PhD, Head of Department of Oncogynecology.

63 14-ya liniya, Rostov-on-Don, 344037; Tel.: +7 (863) 300 02 00, ext. 380

Russian Federation

E. A. Lukbanova

National Medical Research Centre for Oncology

Email: katya.samarskaja@yandex.ru
ORCID iD: 0000-0002-3036-6199

Ekaterina A. Lukbanova - Biologist, Research Fellow, Experimental Laboratory Center.

163 Azovskaya ul., Azov, Rostov Region, 346783, Tel.: +7 (928) 191 45 99

Russian Federation

M. M. Kecheryukova

Rostov State Medical University

Email: adele09161@mail.ru
ORCID iD: 0000-0002-6131-8560

Madina M. Kecheryukova - Postgraduate Student.

29 Nakhichevanskiy pereulok, Rostov-on-Don, 344022; Tel.: +7 (928) 606 37 63

Russian Federation

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Copyright (c) 2020 Maksimov A.Y., Timoshkova M.Y., Verenikina E.V., Lukbanova E.A., Kecheryukova M.M.

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