BENIGN EPITHELIAL NEOPLASIA ASSOCIATED WITH BETA-HUMAN PAPILLOMA VIRUS

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Abstract

Aim: To study an association between acrochordon and human papilloma virus (HPV) using quantitative analysis of viral desoxyribonucleic acid (DNA); to detect different phenotypes of beta-HPV. Materials and methods: We examined 52  patients (22 immuno-suppressed patients and 30 immunocompetent subjects) in the Dermatovenereology and Dermato-Oncology Department and Chronic Dialysis and Kidney Transplantation Department of the Moscow Regional Research and Clinical Institute (MONIKI). Control group included 49 healthy donors. Burr biopsy samples (micro-samples) of acrochordon and intact skin (apper arm) were collected in sterile conditions. After sample procession and DNA isolation using DNK-sorb-C kit (Central Research Institute for Epidemiology – CRIE), polymerase chain reaction for HPV was performed with real-time fluorescent hybridization detection. For DNA amplification and detection we used RotorGene 3000 analyzer (Corbett Research, Australia). In the beta-HPV assay, recombinant plasmids were used as positive controls and control human beta-globin gene fragments (CRIE). 4 oligo-nucleotide systems (group-specific primers and probes) were used for the detection of beta-HPV DNA. Results: Preliminary data indicated that acrochordons of open and covered skin regions were common in renal transplant recipients. Beta-HPV DNA was more frequent in acrochordons and intact skin (64% and 54%) of renal transplant recipients compared to healthy donors (47%). 57% of renal transplant recipients demonstrated mixed infection in acrochordons. Conclusion: HPV DNA was frequently detected in acrochordons and intact skin of renal transplant recipients. In immunocompetent patients prevalence of HPV DNA in acrochordons was significantly higher compared to intact skin.

About the authors

V. A. Molochkov

Moscow Regional Research and Clinical Institute (MONIKI); 61/2 Shchepkina ul., Moscow, 129110, Russian Federation

Email: fake@neicon.ru
MD, PhD, Professor, Head of the Dermatovenereology and Dermato-Oncology Departmen Russian Federation

L. V. Korneva

Moscow Regional Research and Clinical Institute (MONIKI); 61/2 Shchepkina ul., Moscow, 129110, Russian Federation

Author for correspondence.
Email: lvkorneva@mail.ru
MD, PhD, Assistant Professor, Dermatovenereology and Dermato-Oncology Department Russian Federation

E. S. Snarskaya

I.M. Sechenov First Moscow State Medical University; 8/2 Trubetskaya ul., Moscow, 119991, Russian Federation

Email: fake@neicon.ru
MD, PhD, Professor, Department of Skin and Venereal Diseases, Institute for Professional Education Russian Federation

E. O. Shcherbakova

Moscow Regional Research and Clinical Institute (MONIKI); 61/2 Shchepkina ul., Moscow, 129110, Russian Federation

Email: fake@neicon.ru
MD, PhD, Senior Research Associate, Chronic Dialysis and Kidney Transplantation Department Russian Federation

A. A. Polyanskaya

Medical Aesthetics Clinic “Premium Aesthetics”; 3–6 Kazarmennyy pereulok, Moscow, 109028, Russian Federation

Email: fake@neicon.ru
Dermatovenereologist, Cosmetologis Russian Federation

E. K. Nodel’man

Central Research Institute for Epidemiology; 3а Novogireevskaya ul., Moscow, 111123, Russian Federation

Email: fake@neicon.ru
PhD, Junior Research Associate, Molecular Diagnosis and Epidemiology Department Russian Federation

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Copyright (c) 2014 Molochkov V.A., Korneva L.V., Snarskaya E.S., Shcherbakova E.O., Polyanskaya A.A., Nodel’man E.K.

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