DEVELOPMENT OF NON-INVASIVE METHOD FOR BLOOD MICROCIRCULATION DISORDERS DIAGNOSTICS IN DIABETIC PATIENTS USING LASER DOPPLER FLOWMETRY

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Abstract

Background: Pathogenesis of diabetes complications is due to systemic microcirculatory disturbances, while there is a deficit in instrumental methods of objective assessment of the microvasculature. Aim: To provide diagnostics of microcirculatory disorders in patients with diabetes mellitus (DM) using laser Doppler flowmetry. Materials and methods: The study included 11 patients with decompensated type 1 and type 2 DM (HbA1c level – 9.1±2.2%), the duration of the disease being more than 5 years. The control group consisted of 11 healthy volunteers. Blood microcirculation in skin was measured using LAKK-02 system. We examined the condition of blood microcirculation during the functional testing (occlusion, heat, cold, orthostatic) in different combinations and durations of action. For analysis of the quantitative data, the mean values and standard deviations were calculated (M±SD), the differences between the groups were tested using the double-sided Student’s test. Results: The diagnostic tests using a combination of thermal and orthostatic effects were developed. The said diagnostic functional testing and transformation of perfusion units into the relative values of microcirculation (by dividing perfusion index by the average value of microcirculation during the base period) revealed significant differences in microcirculation levels between diabetic patients and the control group: for foot testing, relative microcirculation indices were 3.2±1.9 in the study group and 6.3±4.6 in the control group (p=0.05), for hand testing, the relative indexes were 3.3±1.4 and 5.3±2.8, respectively (p<0.05). Conclusion: The developed method has demonstrated the efficacy of detection of microcirculatory disorders in patients with diabetes. In future, it can be used for the early diagnosis of diabetic vascular complications and as an additional method for monitoring the effectiveness of DM treatment.

About the authors

A. A. Glazkov

Moscow Regional Research and Clinical Institute (MONIKI)
Lomonosov Moscow State University

Author for correspondence.
Email: fake@neicon.ru
Russian Federation

D. A. Kulikov

Moscow Regional Research and Clinical Institute (MONIKI)

Email: fake@neicon.ru
Russian Federation

A. V. Dreval’

Moscow Regional Research and Clinical Institute (MONIKI)

Email: fake@neicon.ru
Russian Federation

Yu. A. Kovaleva

Moscow Regional Research and Clinical Institute (MONIKI)

Email: fake@neicon.ru
Russian Federation

V. I. Shumskii

Moscow Regional Research and Clinical Institute (MONIKI)

Email: fake@neicon.ru
Russian Federation

D. A. Rogatkin

Moscow Regional Research and Clinical Institute (MONIKI)

Email: fake@neicon.ru
Russian Federation

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Copyright (c) 2014 Glazkov A.A., Kulikov D.A., Dreval’ A.V., Kovaleva Y.A., Shumskii V.I., Rogatkin D.A.

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