Specific biological properties of Listeria innocua spp. isolated in Primorye Territory

Cover Page

Cite item


Rationale: Most cases of listeriosis are caused by the pathogenic Listeria monocytogenes. Some cases of isolation of L.  innocua with pathogenicity factors from foods have been published, as well as on the cases of the disease in humans caused by this species. Aim: To assess biological properties including potential pathogenicity of L.  innocua, isolated from food and environmental objects. Materials and methods: We performed microbiological study of L. innocua cultures isolated from foods (n = 35) and environmental objects (n = 15) on the territory of Primorye Territory (Russian Federation), as well as assessment of their sensitivity to antibiotics. Results: The studied L.  innocua cultures showed stable phenotypic features of their biological properties, such as morphology, typical colony growth on the medium with characteristic odor of fermented milk, blue or blue-green luminescence induced by inclined light, presence of catalase activity and absence of the oxidase activity. Only 38 ± 6.9% of L.  innocua demonstrated movements at T 22 °С. L.  innocua cultures did not ferment mannitol (100% of cultures); they degraded ramnose to its acid without gas (70 ± 6.5%) and degraded xylose (42.8 ± 7%). Listeria isolated from vegetables and environmental objects could ferment ramnose (92.8 ± 7.2% of the studied cultures) and xylose (28.5 ± 12.5%) more frequently than L. innocua isolated from meat and fish foods. L.  innocua demonstrated variable biochemical activities towards mannose (92 ± 3.8%), saccharose (85.7 ± 7.8%) and melesitose (76.2 ± 9.5%). L. innocua cultures with hemolytic activity (34 ± 6.7%) (α or β  type) were isolated, more commonly from fish products. All Listeria irrespective of their isolation source showed lipase activity. L.  innocua cultures from foods and environmental objects were highly sensitive to antimicrobials from the following classes: penicillins (ampicillin, carbenicillin, combined amoxicillin and clavulanic acid), aminoglycosides (gentamycin, amikacin), carbapenems (meropenem), and fluoroquinolones (ofloxacin). Conclusion: Some biological properties of L. innocua were variable depending on the source of isolation. Isolation of Listeria with atypical properties in theterritory of Primorye Territory requires that these microorganisms should be studied in more detail. 

About the authors

E. A. Zaitseva

Pacific State Medical University

Author for correspondence.
Email: elza200707@mail.ru

Zaitseva Elena A. – MD, PhD, Professor, Chair of Microbiology and Virology 

2 Ostryakova prospekt, Vladivostok, 690002

Russian Federation


  1. Тартаковский ИС, Малеев ВВ, Ермолаева СА. Листерии: роль в инфекционной патологии человека и лабораторная диагностика. М.: Медицина для всех; 2002. 200 с.
  2. Snapir YM, Vaisbein E, Nassar F. Low virulence but potentially fatal outcome – Listeria ivanovii. Eur J Intern Med. 2006;17(4):286–7. doi: 10.1016/j.ejim.2005.12.006.
  3. Guillet C, Join-Lambert O, Le Monnier A, Leclercq A, Mechaï F, Mamzer-Bruneel MF, Bielecka MK, Scortti M, Disson O, Berche P, Vazquez-Boland J, Lortholary O, Lecuit M. Human listeriosis caused by Listeria ivanovii. Emerg Infect Dis. 2010;16(1):136–8. doi: 10.3201/eid1601.091155.
  4. Rapose A, Lick SD, Ismail N. Listeria grayi bacteremia in a heart transplant recipient. Transpl Infect Dis. 2008;10(6):434–6. doi: 10.1111/j.1399-3062.2008.00333.x.
  5. Salimnia H, Patel D, Lephart PR, Fairfax MR, Chandrasekar PH. Listeria grayi: vancomycin-resistant, gram-positive rod causing bacteremia in a stem cell transplant recipient. Transpl Infect Dis. 2010;12(6):526–8. doi: 10.1111/j.1399-3062.2010.00539.x.
  6. Rocourt J, Hof H, Schrettenbrunner A, Malinverni R, Bille J. Acute purulent Listeria seelingeri meningitis in an immunocompetent adult. Schweiz Med Wochenschr. 1986;116(8):248–51.
  7. Perrin M, Bemer M, Delamare C. Fatal case of Listeria innocua bacteremia. J Clin Microbiol. 2003;41(11):5308–9. doi: 10.1128/JCM.41.11.5308-5309.2003.
  8. Andre P, Genicot A. First isolation of Listeria welshimeri in a human. Zentralbl Bakteriol Mikrobiol Hyg A. 1987;263(4):605–6.
  9. Montazeri N, Himelbloom BH, Oliveira AC, Leigh MB, Crapo CA. Refined liquid smoke: a potential antiListerial additive to coldsmoked sockeye salmon (Oncorhynchus nerka). J Food Prot. 2013;76(5):812–9. doi: 10.4315/0362-028X.JFP-12-368.
  10. Volokhov DV, Duperrier S, Neverov AA, George J, Buchrieser C, Hitchins AD. The presence of the internalin gene in natural atypically hemolytic Listeria innocua strains suggests descent from L. monocytogenes. Appl Environ Microbiol. 2007;73(6):1928–39. doi: 10.1128/AEM.01796-06.
  11. Johnson J, Jinneman K, Stelma G, Smith BG, Lye D, Messer J, Ulaszek J, Evsen L, Gendel S, Bennett RW, Swaminathan B, Pruckler J, Steigerwalt A, Kathariou S, Yildirim S, Volokhov D, Rasooly A, Chizhikov V, Wiedmann M, Fortes E, Duvall RE, Hitchins AD. Natural atypical Listeria innocua strains with Listeria monocytogenes pathogenicity island 1 genes. Appl Environ Microbiol. 2004;70(7):4256–66. doi: 10.1128/AEM.70.7.4256-4266.2004.
  12. El-Shenawy M, El-Shenawy M, Mañes J, Soriano JM. Listeria spp. in Street-Vended Readyto-Eat Foods. Interdiscip Perspect Infect Dis. 2011;2011:968031. doi: 10.1155/2011/968031.
  13. Зайцева ЕА, Ермолаева СА, Пуховская НМ, Мусатов ЮС, Иванов ЛИ, Сомов ГП. Распространение Listeria monocytogenes и ее роль в инфекционной патологии на Дальнем Востоке России. Тихоокеанский медицинский журнал. 2010;(4):19–23.
  14. Clayton EM, Daly KM, Guinane CM, Hill C, Cotter PD, Ross PR. Atypical Listeria innocua strains possess an intact LIPI-3. BMC Microbiol. 2014;14:58. doi: 10.1186/1471-2180-14-58.
  15. Rocha PR, Dalmasso A, Grattarola C, Casalone C, Del Piero F, Bottero MT, Capucchio MT. Atypical cerebral listeriosis associated with Listeria innocua in a beef bull. Res Vet Sci. 2013;94(1): 111–4. doi: 10.1016/j.rvsc.2012.07.017. 16. Fentahun T, Fresebehat A. Listeriosis in small ruminants: a review. Advan Biol Res. 2012;6(6): 202–9. doi: 10.5829/idosi.abr.2012.6.6.66159.
  16. Стародумова СМ, Зайцева ЕА. Способ быстрой идентификации бактерий рода Listeria и патогенного вида Listeria monocytogenes с помощью мультиплексной ПЦР. Тихоокеанский медицинский журнал. 2014;(1):95–7.
  17. El-Shenawy MA. Listeria spp. in the coastal environment of the Aqaba Gulf, Suez Gulf and the Red Sea. Epidemiol Infect. 2006;134(4): 752–7. doi: 10.1017/S0950268805005601.

Supplementary files

There are no supplementary files to display.

Copyright (c) 2017 Zaitseva E.A.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies