CYTOGENETIC EFFECTS OF LOW NEUTRON DOSES IN MAMMALIAN CELLS

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Abstract

Background: Studies of biological effects of low dose radiation are important due to exposure of large patient groups to low radiation doses during therapeutic and diagnostic procedures. Details of biological effects of neutron irradiation have been poorly studied, including cytogenetic effects of its low doses, although they could be important during neutron therapy and for development of normatives on radiation protection with ion accelerators, including therapeutic ones.
Aim: To study patterns of structural chromosomal damage in mammalian cells exposed to 14.5 MeV neutrons at doses up to 1 Gy.
Materials and methods: Cytogenetic effect of 14.5 MeV neutrons was evaluated in human blood lymphocytes and Chinese hamster ovarian cells CHO-K1 in the dose range of 0.1–1.2 Gy, dose rate range 0.3–60 mGy/min, pulse frequency 50, 5, and 0.017 Hz. Chromosome preparations were conventionally prepared. All types of chromosomal aberrations were included into the analysis.
Results: In the 14.5 MeV neutron dose range studied, both cell cultures demonstrated the same dose-effect pattern: a drastic increase of chromosomal aberrations frequency at doses of 0.12–0.15 Gy, with a somewhat lower increase of the number of aberration in the dose range 0.3–0.5 Gy and regular linear dose dependence thereafter. This pattern was found both on the total chromosome aberrations frequency and on dicentric (lymphocytes) and paired fragments (CHO-K1) frequencies.
Conclusion: The results obtained indicate the existence of a phenomenon of hypersensitivity and induced radio-resistance in human lymphocytes and Chinese hamster ovarian cells (CHO-K1) following irradiation with 14.5 MeV neutrons.

About the authors

E. V. Koryakina

A. Tsyb Medical Radiological Research Centre – branch of the National Medical Research Radiological Centre of the Ministry of Health of the Russian Federation, Obninsk, Kaluzhskaya oblast

Author for correspondence.
Email: ek-koryakina@mrrc.obninsk.ru
Koryakina Ekaterina Vladimirovna – PhD (Biol.), Senior Research Fellow, Division of Radiation Biophysics Russian Federation

V. I. Potetnya

A. Tsyb Medical Radiological Research Centre – branch of the National Medical Research Radiological Centre of the Ministry of Health of the Russian Federation, Obninsk, Kaluzhskaya oblast

Email: ek-koryakina@mrrc.obninsk.ru
Potetnya Vladimir Ivanovich – PhD (Biol.), Senior Research Fellow, Division of Radiation Biophysics Russian Federation

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Copyright (c) 2015 Koryakina E.V., Potetnya V.I.

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