Email this article Polymorphism of the adiponutrin gene (PNPLA3) in the indigenous inhabitants of the Republic of Sakha (Yakutia) with type 2 diabetes mellitus
- Authors: Kurtanov K.A.1, Sydykova L.A.2, Pavlova N.I.1, Filippova N.P.1, Dodokhov V.V.1, Apsolikhova G.A.1, Solov'eva N.A.1, D'yakonova A.T.1, Neustroeva L.M.1, Varlamova M.A.1, Borisova N.V.2
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Affiliations:
- Yakut Science Center of Complex Medical Problems
- M.K. Ammosov North-Eastern Federal University
- Issue: Vol 46, No 3 (2018)
- Pages: 258-263
- Section: ARTICLES
- URL: https://almclinmed.ru/jour/article/view/827
- DOI: https://doi.org/10.18786/2072-0505-2018-46-3-258-263
- ID: 827
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Abstract
Rationale: The association of rs738409 I148M polymorphism with type 2 diabetes mellitus (T2DM) and non-alcoholic fatty liver disease has been confirmed for several ethnic and territorial groups. Up to now, no such studies have been performed in the populations of Yakutia.
Aim: To study allele frequency distribution and to identify associations of the PNPLA3 gene polymorphism (rs738409 C>G) with T2DM in the Yakuts.
Materials and methods: DNA samples from 106 T2DM patients were used in the study; the control group included samples from 72 healthy volunteers. All study participants were ethnic Yakuts and were living in the territory of the Republic of Sakha (Yakutia), Russian Federation. rs738409 polymorphism of the PNPLA3 gene was studied by polymerase chain reaction and by restriction fragment length polymorphism.
Results: There were no significant difference in the distribution of the allele frequencies and genotypes of the polymorphous variant of the PNPLA3 gene (rs738409) between the T2DM patients and the healthy control. Both groups showed prevailing allele G (р = 0.01) and homozygous genotype GG (96%).
Conclusion: High frequency of the allele G (74.1%) with predominance of GG genotype (58.5%) was found in type 2 diabetic patients.
About the authors
Kh. A. Kurtanov
Yakut Science Center of Complex Medical Problems
Author for correspondence.
Email: hariton_kurtanov@mail.ru
Khariton A. Kurtanov – MD, PhD, Head of the Department of Molecular Genetics.
32–66 Petrovskogo ul., Yakutsk, 677027, Republic of Sakha (Yakutia), Tel.: +7 (914) 106 00 30
Russian FederationL. A. Sydykova
M.K. Ammosov North-Eastern Federal University
Email: fake@neicon.ru
Lyubov A. Sydykova – MD, PhD, Head of the Chair of Propaedeutic and Faculty Therapy with Endocrinology and Physical Therapy, Medical Institut.
58 Belinskogo ul., Yakutsk, 677000, Republic of Sakha (Yakutia)
Russian FederationN. I. Pavlova
Yakut Science Center of Complex Medical Problems
Email: fake@neicon.ru
Nadezhda I. Pavlova – PhD (in Biology), Head of the Laboratory of Heritable Pathology.
4 Sergelyakhskoe shosse, Yakutsk, 677010, Republic of Sakha (Yakutia)
Russian FederationN. P. Filippova
Yakut Science Center of Complex Medical Problems
Email: fake@neicon.ru
Natal'ya P. Filippova – PhD (in Biology), Associate Professor, Senior Research Fellow, Laboratory of Population Genetics.
4 Sergelyakhskoe shosse, Yakutsk, 677010, Republic of Sakha (Yakutia)
Russian FederationV. V. Dodokhov
Yakut Science Center of Complex Medical Problems
Email: fake@neicon.ru
Vladimir V. Dodokhov – PhD (in Biology), Research Fellow, Laboratory of Population Genetics.
4 Sergelyakhskoe shosse, Yakutsk, 677010, Republic of Sakha (Yakutia)
Russian FederationG. A. Apsolikhova
Yakut Science Center of Complex Medical Problems
Email: fake@neicon.ru
Galina A. Apsolikhova – Junior Research Fellow, Laboratory of Heritable Pathology.
4 Sergelyakhskoe shosse, Yakutsk, 677010, Republic of Sakha (Yakutia)
Russian FederationN. A. Solov'eva
Yakut Science Center of Complex Medical Problems
Email: fake@neicon.ru
Natal'ya A. Solov'eva – MD, PhD, Head of the Laboratory of Population Genetics.
4 Sergelyakhskoe shosse, Yakutsk, 677010, Republic of Sakha (Yakutia)
Russian FederationA. T. D'yakonova
Yakut Science Center of Complex Medical Problems
Email: fake@neicon.ru
Aleksandra T. D'yakonova – Junior Research Fellow, Laboratory of Heritable Pathology.
4 Sergelyakhskoe shosse, Yakutsk, 677010, Republic of Sakha (Yakutia)
Russian FederationL. M. Neustroeva
Yakut Science Center of Complex Medical Problems
Email: fake@neicon.ru
Lena M. Neustroeva – Junior Research Fellow, Laboratory of Population Genetics.
4 Sergelyakhskoe shosse, Yakutsk, 677010, Republic of Sakha (Yakutia)
Russian FederationM. A. Varlamova
Yakut Science Center of Complex Medical Problems
Email: fake@neicon.ru
Marina A. Varlamova – Junior Research Fellow, Laboratory of Heritable Pathology.
4 Sergelyakhskoe shosse, Yakutsk, 677010, Republic of Sakha (Yakutia)
Russian FederationN. V. Borisova
M.K. Ammosov North-Eastern Federal University
Email: fake@neicon.ru
Natal'ya V. Borisova – MD, PhD, Professor, Chair of Physiology, Medical Institut.
58 Belinskogo ul., Yakutsk, 677000, Republic of Sakha (Yakutia)
Russian FederationReferences
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