Email this article Changes in plasma spectral characteristics during the in vitro contact of human venous blood with granulated sorbents
- Authors: Burkova N.V.1, Kirichuk O.P.1,2, Romanchuk E.V.2, Davankov V.A.3, Postnov V.N.1,4, Kuznetsov S.I.1
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Affiliations:
- Almazov National Medical Research Centre
- Peter the Great St. Petersburg Polytechnic University
- A.N. Nesmeyanov Institute of Organoelement Compounds of Russian Academy of Sciences
- St. Petersburg State University (Institute of Chemistry)
- Issue: Vol 46, No 8 (2018)
- Pages: 772-777
- Section: ARTICLES
- URL: https://almclinmed.ru/jour/article/view/938
- DOI: https://doi.org/10.18786/2072-0505-2018-46-8-772-777
- ID: 938
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Abstract
Rationale: During hemosorption procedures, it is important to investigate not only the sorption and activation characteristics of hemocontact agents, but also to assess the effect of sorbents on the parameters of blood homeostasis. The intensity of hemolysis can be judged by the degree of changes in optical density of blood plasma at wavelengths corresponding to the peak absorption of hemoglobin (414, 544 and 577 nm).
Aim: To assess the effect of three granular sorbents (SKT-6A, HPS, Silochrome C-120) contacting human venous blood in vitro on changes in plasma spectral characteristics.
Materials and methods: The blood contact was modeled at bench conditions with the use of donated blood at rotating mode. Blood samples were drawn before the experiment and after 5, 20, 40, and 60 minutes. Spectroscopic assessment was performed in the visible light range (300–700 nm) with UNICO 2802(S) spectrophotometer.
Results: The interaction of the SKT-6A sorbent with blood resulted in a 17.3% decrease in the plasma optical density at a wavelength of 540 nm, compared to baseline, as soon as at 5 minute of the experiment (p < 0.05). The decline in optical density imposed by the blood contact with HPS ranged from 2.6 to 12.1% (p < 0.05) during the observation period. The sorption activity of SKT-6A and HPS prevailed over their lytic properties. On the contrary, the percentage change in the optical density of the Silochrome C-120 sorbent during its blood contact increased from 25.6 to 38.3% (p < 0.05), indicating that this sorbent was inducing hemolysis. The sorbents tested can be arranged as follows according to their ability to induce hemolysis during their contact with blood: HPS < SKT-6A < Silochrome C-120.
Conclusion: The tested SKT-6A and HPS sorbents can be used as blood-contact agents for the low volume hemoperfusion. The HPS agent seems to be the most promising for routine clinical use. The Silochrome C-120 sorbent requires some chemical modification to improve its properties of hemocompatibility.
About the authors
N. V. Burkova
Almazov National Medical Research Centre
Author for correspondence.
Email: n.burk@list.ru
Natal'ya V. Burkova – PhD, ScD in Biology, Associate Professor, Professor of Medical Faculty, Institute of Medical Education; Leading Research Fellow, Research Laboratory for Bioprosthetics and Cardioprotection, Center of Experimental Biomodeling, Institute of Experimental Medicine
15 Parkhomenko prospekt, Saint Petersburg, 194156
Russian FederationO. P. Kirichuk
Almazov National Medical Research Centre;Peter the Great St. Petersburg Polytechnic University
Email: fake@neicon.ru
Oksana P. Kirichuk – Assistant Researcher, Laboratory of Bioprosthetics and Cardioprotection, Center of Experimental Biomodeling, Institute of Experimental Medicine Almazov National Medical Research Centre; Student, Chair of Medical Physics Peter the Great St. Petersburg Polytechnic University
2 Akkuratova ul., Saint Petersburg, 197341,
29 Politekhnicheskaya ul., Saint Petersburg, 195251
Russian FederationE. V. Romanchuk
Peter the Great St. Petersburg Polytechnic University
Email: fake@neicon.ru
Elizaveta V. Romanchuk – Student, Chair of Medical Physics
29 Politekhnicheskaya ul., Saint Petersburg, 195251
Russian FederationV. A. Davankov
A.N. Nesmeyanov Institute of Organoelement Compounds of Russian Academy of Sciences
Email: fake@neicon.ru
Vadim A. Davankov – PhD, ScD in Chemistry. Sci., Professor, Head of the Laboratory of Stereochemistry of Sorption Processes
28 Vavilovа ul., Moscow, 119334
Russian Federation
V. N. Postnov
Almazov National Medical Research Centre;St. Petersburg State University (Institute of Chemistry)
Email: fake@neicon.ru
Victor N. Postnov – PhD in Chemistry. Sci., Associate Professor, Senior Research Fellow, Research Laboratory of Nanotechnologies, Center of Experimental Biomodeling, Institute of Experimental Medicine Almazov National Medical Research Centre; Associate Professor, Chair of Solid State Chemistry St. Petersburg State University (Institute of Chemistry)
2 Akkuratova ul., Saint Petersburg, 197341,
26 Universitetskiy prospekt, Peterhof, Saint Petersburg, 198504
Russian FederationS. I. Kuznetsov
Almazov National Medical Research Centre
Email: fake@neicon.ru
Sergey I. Kuznetsov – MD, PhD, Professor, Chief Research Fellow, Research Laboratory for Bioprosthetics and Cardioprotection, Center of Experimental Biomodeling, Institute of Experimental Medicine
2 Akkuratova ul., Saint Petersburg, 197341
Russian FederationReferences
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